PHYSICS/BK21 Colloquium
관련링크
본문
Dynamic Force-Fluorescence Spectroscopy of a DNA Holliday Junction Reveals a
Conformational Reaction
연사: size=3>홍성철[서울대]
size=3>일시: 2007 년 5 월 11 일 오후 3시
장소: 무은재 기념관 307호 (멀티미디어 강의실)
ABSTRACT:
being recognized as important determinants of many biological processes,
single-molecule manipulation techniques have been continually evolving to break
new records in their sensitivity and stability 1-3. However, one biologically
relevant detection regime that has been out of reach is that of high spatial
resolution under weak applied forces. We have therefore developed a new
single-molecule technique, whereby mechanical manipulation via optical tweezers
and observation of molecular motion via single-molecule FRET are combined
without compromising their individual capabilities. Using this approach we have
studied the conformational dynamics of the Holliday junction under applied
stretching force, that has revealed the existence a hidden intermediate state
along the reaction coordinate. The length of helical arms is shown to be an
important factor determining force sensitivity of the Holliday junction. This
suggests that the recombination process occurring in the cell could be extremely
sensitive to sub-pico Newton forces that last only milliseconds.
size=3>문의처: 지승훈(054-279-2094, href=mailto:jhish@postech.ac.kr>jhish@postech.ac.kr)
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