물리학과 특별세미나 개최 안내
물리학과에서는 특별세미나를 아래와 같이 개최하오니 관심있는 분들의 많은 참석을 바랍니다.
1. 연사: 강준호 박사 (KIST)
2. 일시: 2022. 11. 14(월), 오후 4시 30분
3. 장소: 공학3동 302호 (세미나실)
4. 제목: Physical dynamics of single cells
Physical properties of cells, such as mass, volume, and stiffness are valuable indicators of cell state and function. Numerous cellular processes including cell development, differentiation, migration, and disease progression (e.g., cancer metastasis) accompany changes in cellular physical properties. However, studying the physical dynamics is challenging because both the magnitude and duration of cellular changes are heterogeneous across the cell population. In this talk, we present new methods to monitor single-cell stiffness, bioenergetics, and mass over the entire cell cycle with a temporal resolution of ~1 min.
We utilize the suspended microchannel resonator (SMR), a fluid-filled cantilever capable of measuring the buoyant mass of a cell by the change of SMR resonant frequency. First, we quantify cell stiffness via acoustic scattering of waves from a cell inside the SMR. Through simulations, experiments with hydrogels, and chemical perturbation of cells, we show that our readout from acoustic scattering measures stiffness1. Second, we monitor the mitochondrial membrane potential of single mouse lymphocytic leukemia L1210 cells and model ATP synthesis dynamics using an electrical circuit model of mitochondria2. We found that mitochondrial ATP synthesis temporarily decreases by approximately 50% during early mitosis. Lastly, we monitored L1210 cell growth during normal proliferation and polyploidization to show that increasing cell mass by over 100-fold does not limit maximal growth efficiency, indicating exponential growth3. Altogether, these studies revealed physical dynamics and their underlying mechanism throughout the cell cycle that have not been observed by endpoint assays.
[1] Kang, et al. “Non-invasive monitoring of single-cell mechanics by acoustic scattering”. Nature Methods (2019).
[2] Kang*, Katsikis*, et al. “Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division”. Nature Communications (2020).
[3] Mu*, Kang*, et al. “Mass measurements during lymphocytic leukemia cell polyploidization decouple cell cycle-and cell size-dependent growth”. Proceedings of the National Academy of Sciences (2020).
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